Effect of sodium polyanethol sulfonate in blood cultures

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Effect of blood dilution on recovery of organisms from clinical blood cultures in medium containing sodium polyanethol sulfonate.

This clinical study was designed to evaluate the standard laboratory protocol that requires blood specimens be diluted with greater than or equal to 10 volumes of media. Blood was collected from hospitalized patients, and 1 ml was inoculated into each of three vials containing 2.3, 7.3, and 24 ml of BACTEC 6B aerobic medium resulting in dilutions of 1:4, 1:10, and 1:30, respectively. The three ...

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Sodium polyanethol sulfonate sensitivity of anaerobic cocci.

Growth of Peptostreptococcus anaerobius was shown to be totally inhibited by sodium polyanethol sulfonate (SPS). Other anaerobic cocci grew in the presence of SPS although some strains of Peptococcus prevotii and Peptococcus magnus showed delayed growth. A SPS disk assay for the presumptive identification of P. anaerobius is described.

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Inhibitory effect in vitro of sodium polyanethol sulfonate on the growth of Neisseria meningitidis.

The influence of 0.05% sodium polyanethol sulfonate on the growth of 24 strains of Neisseria meningitidis in broth medium was examined; several of the strains were markedly inhibited. A paper disk method was evolved for screening the sensitivity of various bacteria to sodium polyanethol sulfonate on solid medium; the highest sensitivity was observed in N. meningitidis and Neisseria gonorrhoeae....

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Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media.

The inhibitory effect of sodium polyanethol sulfonate (0.05%) upon growth of Neisseria meningitidis was found to be neutralized by adding gelatin (l.1%) to the growth medium. The neutralizing effect was demonstrated in solid medium, as well as in nutrient broth for blood cultures. The findings parallel those of Wilkins and West (6) regarding gelatin neutralization of the inhibitory effect of so...

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Neutralization of human serum lysozyme by sodium polyanethol sulfonate but not by sodium amylosulfate.

Sodium polyanethol sulfonate (SPS) at 500 microgram/ml, but not sodium amylosulfate (SAS) at 500 microgram/ml, precipitated egg white lysozyme (1 mg and 50 microgram of lysozyme per ml) as determined with the assay strain Micrococcus lysodeikticus ATCC 4698. Fresh and heat-inactivated (56 degrees C, 30 min) human serum (80%, vol/vol) killed M. lysodeikticus (10(4) bacteria per ml at zero time) ...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1975

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.1.2.119-123.1975